Thermo Fisher Scientific ALKBH5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ALKBH5(Accession Q6P6C2), corresponding to amino acid residues R286-R336. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ALKBH5,uniProtId:Q6P6C2-2,ncbiNodeId:9606,antigenRange:286-336,antigenLength:394,antigenImageFileName:PA5-100873_ALKBH5_Q6P6C2-2_Rabbit.svg,antigenImageFileNamePDP:PA5-100873_ALKBH5_Q6P6C2-2_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2850370

Product Specific Information

Antibody detects endogenous levels of total ALKBH5.

Target Information

ALKBH5 (RNA demehtylase ALKBH5) is a dioxygenase that demethylates RNA by oxidative demethylation. Specifically, it demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Demethylation of m6A mRNA affects mRNA processing and export. ALKBH5 can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). It requires molecular oxygen, alpha-ketoglutarate and iron.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.