Thermo Fisher Scientific TurboLuc luciferase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Species Reactivity

Luciferase

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified recombinant TurboLuc luciferase expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539909

Product Specific Information

Western blot analysis of PA1-178 detects an ~16-18 kDa protein in Tluc expressing cells.

PA1-178 can be used for activity-independent verification of Tluc expression in qIP assays.

Target Information

TurboLuc™ (Tluc) is a novel intracellular luciferase derived from the marine copepod Metridia luciferase family. The Tluc gene was developed using directed mutagenesis for optimal performance and codon optimized for mammalian cell expression. Tluc is an ATP-independent enzyme that utilizes the substrate coelenterazine to produce intense bioluminescence light. Tluc is smaller and brighter than other commonly used luciferases, including firefly and Renilla. The intense bioluminescence greatly enhances sensitivity of Tluc luciferase-based assays, enabling detection of very minute amounts of luciferase (signal) in very sensitive assays such as protein interaction qIP assays (Prod # 82032, 82033, 82035, 82036).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.