Thermo Fisher Scientific TurboLuc luciferase Polyclonal Antibody
TurboLuc luciferase를 인식하는 토끼 폴리클로날 항체로, Western blot에서 1:1,000 희석으로 사용 가능. ATP 비의존적 TurboLuc 발현 확인에 적합하며, 높은 감도와 특이성을 제공. 연구용으로만 사용.
- 카탈로그번호
- PA1178
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific TurboLuc luciferase Polyclonal Antibody
Applications
- Western Blot (WB)
Tested Dilution: 1:1,000
Publications: - [#references-component-id]
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Luciferase |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified recombinant TurboLuc luciferase expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539909 |
Product Specific Information
Western blot analysis of PA1-178 detects an ~16–18 kDa protein in TurboLuc (Tluc) expressing cells.
PA1-178 can be used for activity-independent verification of Tluc expression in qIP assays.
Target Information
TurboLuc™ (Tluc) is a novel intracellular luciferase derived from the marine copepod Metridia luciferase family.
The Tluc gene was developed using directed mutagenesis for optimal performance and codon optimized for mammalian cell expression.
Tluc is an ATP-independent enzyme that utilizes the substrate coelenterazine to produce intense bioluminescence light.
Compared to firefly and Renilla luciferases, Tluc is smaller and brighter, providing enhanced sensitivity for luciferase-based assays.
The strong bioluminescence enables detection of minute amounts of luciferase in sensitive assays such as protein interaction qIP assays (Prod #82032, 82033, 82035, 82036).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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