Thermo Fisher Scientific ERp57 Recombinant Rabbit Monoclonal Antibody (JM11-44)
ERp57 단백질을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 감도를 제공. Western blot, IHC, IP 등 다양한 응용 가능. HEK293 세포에서 발현되며, 단백질 A로 정제된 액상 형태. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM11-44 |
| Immunogen | Synthetic peptide within Human ERp57 aa 175–208 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809899 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences are cloned from immunoreactive rabbits, and individual clones are screened to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
ERp57 is an endoplasmic reticulum protein that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins.
Although previously considered a phospholipase, it has been shown to possess protein disulfide isomerase activity. Complexes of lectins and ERp57 promote protein folding by facilitating disulfide bond formation in glycoprotein substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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