Thermo Fisher Scientific TNFRSF11B Polyclonal Antibody, Biotin, PeproTech

Applications

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
METFPPKYLH YDEETSHQLL CDKCPPGTYL KQHCTAKWKT VCAPCPDHYY TDSWHTSDEC LYCSPVCKEL QYVKQECNRT HNRVCECKEG RYLEIEFCLK HRSCPPGFGV VQAGTPERNT VCKRCPDGFF SNETSSKAPC RKHTNCSVFG LLLTQKGNAT HDNICSGNSE STQK

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human OPG. Anti-Human OPG-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human OPG by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human OPG (500-P149) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human OPG.

Western Blot:
To detect hOPG by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hOPG is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P149BT-1MG will be provided as 2 × 500 µg.

Target Information

The protein encoded by this gene is a member of the TNF-receptor superfamily. It is an osteoblast-secreted decoy receptor functioning as a negative regulator of bone resorption. This protein binds specifically to its ligand, osteoprotegerin ligand, and both are key extracellular regulators of osteoclast development. Studies suggest that this protein and its ligand also play roles in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.