Thermo Fisher Scientific RANK (soluble) Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific RANK (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MQIAPPCTSE KHYEHLGRCC NKCEPGKYMS SKCTTTSDSV CLPCGPDEYL DSWNEEDKCL LHKVCDTGKA LVAVVAGNST TPRRCACTAG YHWSQDCECC RRNTECAPGL GAQHPLQLNK DTVCKPCLAG YFSDAFSSTD KCRPWTNCTF LGKRVEHHGT EKSDAVCSSS LPARK.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sRANK Receptor. Anti-Human sRANK Receptor-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human sRANK Receptor by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sRANK Receptor (500-P144) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human sRANK Receptor.

Western Blot:
To detect hsRANK Receptor by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hsRANK Receptor is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P144BT-1MG will be provided as 2 × 500 µg.

Target Information

RANK is a member of the tumor necrosis factor receptor (TNFR) family. The full-length mouse RANK cDNA encodes a type I transmembrane protein with extracellular and cytoplasmic domains. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other TNFR family members in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지