Thermo Fisher Scientific IL-15 Polyclonal Antibody, PeproTech
인간 IL-15 단백질을 특이적으로 인식하는 토끼 폴리클로날 항체입니다. Western blot, ELISA 등 다양한 면역분석에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 세포 활성 및 면역 반응 연구용으로 권장됩니다.
- 카탈로그번호
- 500-P15-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-15 Polyclonal Antibody, PeproTech
Thermo Fisher Scientific IL-15 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 1 publication |
| In vitro Assay (IV) | – | View 1 publication |
| Immunostaining (IS) | – | View 2 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived Recombinant Human IL-15 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929489 |
Product Specific Information
AA Sequence of recombinant protein:
MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-15. Anti-Human IL-15-specific antibody was purified by affinity chromatography employing an immobilized Human IL-15 matrix.
Sandwich ELISA:
For detection of Human IL-15 by sandwich ELISA (using 100 µL/well antibody solution), use 0.5–2.0 µg/mL of this antibody. When combined with PeproTech Biotinylated Anti-Human IL-15 (500-P15Bt) as the detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Human IL-15.
Western Blot:
For detection of Human IL-15 by Western blot, use 0.1–0.2 µg/mL. In conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-15 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
IL-15 (Interleukin 15) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities, binding common hematopoietin receptor subunits and potentially competing for the same receptor, thereby regulating each other’s activity.
It influences CD8+ memory cell balance, activates JAK kinases and transcription factors STAT3, STAT5, and STAT6. Studies in mice suggest IL-15 may enhance expression of apoptosis inhibitor Bcl-xL via STAT6 activation, preventing apoptosis.
IL-15 plays a key role in the growth and differentiation of T and B lymphocytes, NK cells, macrophages, and monocytes, and is essential in immune responses, graft rejection, and autoimmune disease pathogenesis. It is also implicated in inflammatory disorders such as rheumatoid arthritis, psoriasis, and pulmonary inflammation. IL-15 neutralization shows potential therapeutic benefits in psoriasis and diabetes models.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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