Thermo Fisher Scientific IL-15 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

-

In vitro Assay (IV)

-

View 1 publication 1 publication

Immunostaining (IS)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human IL-15 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-15,uniProtId:P40933-1,ncbiNodeId:9606,antigenRange:49-162,antigenLength:162,antigenImageFileName:500-P15-100UG_IL-15_P40933-1_Rabbit.svg,antigenImageFileNamePDP:500-P15-100UG_IL-15_P40933-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929489

Product Specific Information

AA Sequence of recombinant protein: MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-15. Anti-Human IL-15-specific antibody was purified by affinity chromatography employing an immobilized Human IL-15 matrix.

Sandwich ELISA: To detect Human IL-15 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-15 (500-P15Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-15.

Western Blot: To detect Human IL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-15 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other`s activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.