Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), Biotin, eBioscience
Mouse PD-1 (CD279) 인식하는 J43 클론의 비오틴 결합 단일클론 항체로, Flow Cytometry에 최적화되어 있습니다. 면역관용 및 자가면역 연구에 적합하며, 0.25 µg/test로 사용 권장됩니다. PBS 버퍼에 보관하며 4°C에서 암소 저장합니다.
- 카탈로그번호
- 13-9985-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), Biotin, eBioscience
Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 38 publications |
| ELISA | - | View 1 publication |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | J43 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467023 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 786
- eFluor 450
- eFluor 506
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation available
Product Specific Information
The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. PD-L1 (B7-H1) and PD-L2 (B7-DC) are identified as PD-1 ligands, showing overlapping functions and expression on normal tissues and activated antigen-presenting cells.
J43 inhibits the binding of mouse PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells. In vivo, J43 (intact or Fab) enhances contact hypersensitivity and exacerbates acute GVHD, EAE, and NOD diabetes, similar to PD-1-deficient cells or antibodies to PD-L1/PD-L2.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of Con A-stimulated mouse splenocytes and PD-1 transfected cells.
Recommended usage ≤ 0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.
Target Information
Cell-mediated immune responses are initiated by T lymphocytes stimulated by peptides bound to MHC molecules on antigen-presenting cells (APC). Activated T cells express inhibitory receptors such as PD-1 (PDCD-1), which binds ligands PD-L1 and PD-L2 to suppress immune activation. PD-1 signaling prevents overactivation in the absence of danger signals (e.g., LPS). PD-1-deficient mice exhibit hyperactivated immune systems and autoimmune disorders. PD-1 often migrates at higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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