Thermo Fisher Scientific CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 661
• eFluor 450
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.

Applications Reported:
This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.

Applications Tested:
This antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. Binding of this antibody to red cells at high antibody concentration causes cell agglutination. Recommended usage is ≤0.125 µg per test (100 µL final volume). Cell number may vary between 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal assay performance.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD235a (Glycophorin A, GPA) is a single-pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. It carries blood group M and N specificities and provides a mucin-like surface that minimizes aggregation between red blood cells. CD235a also acts as a receptor for Sandei virus, parvovirus, and Streptococcus adhesin Hsa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.