Thermo Fisher Scientific NEK3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human NEK3. Recombinant protein control fragment (Product #RP-91443). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NEK3,uniProtId:P51956-1,ncbiNodeId:9606,antigenRange:209-309,antigenLength:506,antigenImageFileName:PA5-53911_NEK3_P51956-1_Rabbit.svg,antigenImageFileNamePDP:PA5-53911_NEK3_P51956-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2644604

Product Specific Information

Immunogen sequence: NLILKVCQGC ISPLPSHYSY ELQFLVKQMF KRNPSHRPSA TTLLSRGIVA RLVQKCLPPE IIMEYGEEVL EEIKNSKHNT PRKKTNPSRI RIALGNEAST V

Highest antigen sequence identity to the following orthologs: Mouse - 64%, Rat - 60%.

Target Information

NIMA was originally shown in Aspergillus nidulans to be necessary for entry into mitosis. NIMA-related mammalian proteins have since been identified as Nek1, Nek2, Nek3 and Nek4 (also designated STK2 or NRK2). High expression of Nek1 is seen in male and female germ cell lines of mouse. Nek2 is the closest known mammalian relative to NIMA. Like NIMA, Nek2 expression peaks at the G2 to M phase transition. Nek3 is a predominantly cytoplasmic enzyme that was detectable in all organs studied. Levels of Nek3 seem to remain unchanged throughout the cell cycle, but appear to be elevated in G0-arrested, quiescent fibroblasts. In developing testicular germ cells, differential patterns of expression were seen for Nek1, Nek2 and Nek4, indicating possible overlapping, but non-identical functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.