Thermo Fisher Scientific NEK3 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Immunogen sequence:
NLILKVCQGC ISPLPSHYSY ELQFLVKQMF KRNPSHRPSA TTLLSRGIVA RLVQKCLPPE IIMEYGEEVL EEIKNSKHNT PRKKTNPSRI RIALGNEAST V

Sequence identity to orthologs:

• Mouse: 64%
• Rat: 60%

Target Information

NIMA was originally shown in Aspergillus nidulans to be necessary for entry into mitosis. NIMA-related mammalian proteins have since been identified as Nek1, Nek2, Nek3, and Nek4 (also designated STK2 or NRK2).
High expression of Nek1 is seen in male and female germ cell lines of mouse. Nek2 is the closest known mammalian relative to NIMA, and its expression peaks at the G2 to M phase transition.
Nek3 is a predominantly cytoplasmic enzyme detectable in all organs studied. Levels of Nek3 remain unchanged throughout the cell cycle but are elevated in G0-arrested, quiescent fibroblasts. In developing testicular germ cells, differential expression patterns of Nek1, Nek2, and Nek4 indicate possible overlapping but distinct functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.