Thermo Fisher Scientific NEK3 Polyclonal Antibody
Human NEK3 단백질을 인식하는 Rabbit Polyclonal Antibody. IHC(P)에서 1:50–1:200 희석으로 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 항체로, PBS/glycerol buffer에 보존. 연구용으로만 사용.
- 판매단위
- pk
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NEK3 (Product #RP-91443) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2644604 |
Product Specific Information
Immunogen sequence:
NLILKVCQGC ISPLPSHYSY ELQFLVKQMF KRNPSHRPSA TTLLSRGIVA RLVQKCLPPE IIMEYGEEVL EEIKNSKHNT PRKKTNPSRI RIALGNEAST V
Sequence identity to orthologs:
- Mouse: 64%
- Rat: 60%
Target Information
NIMA was originally shown in Aspergillus nidulans to be necessary for entry into mitosis. NIMA-related mammalian proteins have since been identified as Nek1, Nek2, Nek3, and Nek4 (also designated STK2 or NRK2).
High expression of Nek1 is seen in male and female germ cell lines of mouse. Nek2 is the closest known mammalian relative to NIMA, and its expression peaks at the G2 to M phase transition.
Nek3 is a predominantly cytoplasmic enzyme detectable in all organs studied. Levels of Nek3 remain unchanged throughout the cell cycle but are elevated in G0-arrested, quiescent fibroblasts. In developing testicular germ cells, differential expression patterns of Nek1, Nek2, and Nek4 indicate possible overlapping but distinct functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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