Thermo Fisher Scientific LEFTY A Monoclonal Antibody (7C5G1H6H10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

ELISA (ELISA)

1:5,000

-

Immunoprecipitation (IP)

1:2,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

7C5G1H6H10

Immunogen

A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.

Target Information

Left-right determination factor 1 and 2 (Lefty1/2) belong to the TGF-beta superfamily of proteins and are responsible for determination of left-right positioning of visceral organs during embryogenesis. Lefty1 acts as a regulator of Lefty2 and NODAL as the lack of the former resulted in bilateral expression of nodal, lefty2 and Pitx2 (a homeobox gene normally expressed on the left side and blocks the signal for leftness). Lefty2 may play a role in endometrial bleeding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.