Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (29E4.G7)
DYKDDDDK(FALG) 태그 인식용 mouse monoclonal 항체로, WB, IHC, ELISA, IP 등에 사용 가능. 단백질 발현 및 정제, 위치 분석 연구에 적합. FLAG 태그 단백질 검출에 높은 특이성과 재현성을 제공. -20°C 보관, Protein A 정제.
- 카탈로그번호
- 200-301-383x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (29E4.G7)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:20,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:150,000–1:250,000 |
| Immunoprecipitation (IP) | 1:2,000–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 29E4.G7 |
| Immunogen | Synthetic peptide corresponding to the FLAG™ epitope tag DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Glycine and cysteine residues were added to the termini to facilitate coupling. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Available Conjugated Formats
- Agarose
- Biotin
- DyLight 405
- DyLight 488
- DyLight 549
- DyLight 649
- DyLight 680
- DyLight 800
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This purified monoclonal antibody is directed against the FLAG™ motif and is useful for detecting the presence of the epitope tag at either the amino or carboxy terminus of recombinant proteins. It specifically detects overexpressed proteins containing the FLAG™ tag and does not cross-react with endogenous proteins in bacterial extracts.
Target Information
The DYKDDDDK tag (FLAG tag) is a short peptide sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) commonly used as a protein tag to facilitate purification, detection, and localization. It can be fused to either the N- or C-terminus of a protein, allowing easy identification and isolation using anti-FLAG antibodies. The FLAG tag is hydrophilic, minimizing interference with protein structure and function. It is widely used in molecular biology and biochemistry for protein-protein interaction studies, expression analysis, and localization experiments.
FLAG™ is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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