Thermo Fisher Scientific Phospho-JNK1/JNK2/JNK3 (Thr183, Tyr185) Polyclonal Antibody
Phospho-JNK1/JNK2/JNK3 (Thr183, Tyr185) 항체로 사람, 생쥐, 랫트 시료에 반응. Western blot, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체. 세포 신호전달 및 스트레스 반응 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Threonine 183 and Tyrosine 185 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553705 |
Product Specific Information
- Detects endogenous protein at molecular weights of 46 and 54 kDa.
- Purity >95% by SDS-PAGE.
Target Information
C-Jun N-terminal Kinase (JNK), also known as Mitogen-activated protein kinase (MAPK8), belongs to the MAPK superfamily of stress-activated protein kinases. These serine-threonine protein kinases are activated by various extracellular stimuli and mediate signal transduction from the cell surface to the nucleus.
JNK participates in cellular processes such as proliferation, differentiation, transcription regulation, and development. It is activated by stress and inflammatory signals and expressed as ten isoforms due to differential mRNA splicing, with JNK1 and JNK2 being predominant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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