Thermo Fisher Scientific Phospho-MiTF (Ser180) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:150

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human MITF around the phosphorylation site of Serine 180 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MiTF,uniProtId:O75030-1,ncbiNodeId:9606,antigenRange:180,antigenLength:526,antigenImageFileName:PA5-36755_MiTF_O75030-1_Rabbit.svg,antigenImageFileNamePDP:PA5-36755_MiTF_O75030-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2553706

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 59 kDa.

Purity is >95% by SDS-PAGE.

Target Information

Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.