Thermo Fisher Scientific CD154 (CD40 Ligand) Recombinant Rabbit Monoclonal Antibody (JM11-34)
CD154(CD40 Ligand)을 인식하는 토끼 단일클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 적합합니다. HEK293 발현 시스템에서 생산된 재조합 항체로 높은 특이성과 일관성을 제공합니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM11-34 |
| Immunogen | Synthetic peptide within Human CD40L aa 103–150 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809896 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
CD40 Ligand (CD40-L, CD154) is a membrane glycoprotein expressed on the surface of T cells.
It:
- Stimulates B cell proliferation and immunoglobulin secretion in the presence of cytokines
- Induces cytokine production and tumoricidal activity in monocytes
- Co-stimulates proliferation of activated T cells, accompanied by IFN-γ, TNF-α, and IL-2 production
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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