Thermo Fisher Scientific EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody (GFR1195)
EGFR 단백질을 인식하는 Mouse monoclonal antibody로, IHC, ICC/IF, Peptide Array 등 다양한 실험에 사용 가능. 인간 시료 반응성, Protein A/G 정제, 4°C 보관. 암 관련 EGFR 연구용으로 적합.
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Thermo Fisher Scientific EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody (GFR1195)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–4 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GFR1195 |
| Immunogen | Recombinant extracellular domain of human EGFR (aa25–645) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
EGFR (Epidermal Growth Factor Receptor) is a receptor tyrosine kinase that signals in response to various growth factors. Overexpression is associated with numerous cancer types, making EGFR a key therapeutic target.
EGFR is encoded by the EGFR gene on chromosome 7 in humans and belongs to the HER/ERbB family, which includes ERbB2, ERbB3, and ERbB4.
Binding of ligands such as EGF and TGF-α to the extracellular domain induces dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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