Thermo Fisher Scientific EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody (GFR1195)
EGFR 단백질을 인식하는 마우스 단클론 항체로, 면역조직화학(IHC), 면역세포화학(ICC/IF), 펩타이드 배열 분석에 사용 가능. 인간 EGFR에 반응하며, 단백질 A/G로 정제된 액상 형태. 암 연구 및 성장인자 신호 연구용으로 적합.
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Thermo Fisher Scientific EGFR Monoclonal Antibody (GFR1195)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–4 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GFR1195 |
| Immunogen | Recombinant extracellular domain of human EGFR (aa25–645) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris Buffer with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
EGFR (Epidermal Growth Factor Receptor) is a receptor tyrosine kinase that mediates cellular signaling in response to growth factors. Overexpression of EGFR has been associated with various cancers and is the target of biological and small-molecule therapeutics.
The EGFR gene is located on chromosome 7 in humans and belongs to the HER/ERbB family, which includes ERbB2, ERbB3, and ERbB4.
EGFR is a transmembrane receptor; ligand binding (e.g., EGF, TGF-α) induces dimerization and autophosphorylation of cytoplasmic tyrosine residues. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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