Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioSN4 (SN4 C3-3A2)), FITC, eBioscience
인간 CD9 단백질을 인식하는 마우스 단클론 항체로, FITC 형광 표지되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. PBS/BSA 완충액에 보관하며, 4°C 암소 보관 권장. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 9 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioSN4 (SN4 C3-3A2) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10698007 |
Additional Formats
- Unconjugated
- APC
- Biotin
- PE
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 645
- Custom conjugation available
Product Specific Information
Description:
The eBioSN4 monoclonal antibody reacts with human CD9, a 24 kDa tetraspanin family protein characterized by four hydrophobic transmembrane domains. CD9 is expressed in platelets, eosinophils, basophils, pre-B cells, activated T cells, and neural cell lines. Its expression is associated with malignant phenotypes, including 90% of non-T cell acute lymphoblastic leukemia and 50% of chronic lymphocytic and acute myeloblastic leukemias.
In platelets, CD9 is localized in alpha-granules and interacts with integrin αIIb/βIII, contributing to platelet aggregation. CD9 also promotes aggregation of pre-B cell lines and mediates adhesion and migration of pre-B and Schwann cells. It can co-stimulate T cells independently of CD28. The eBioSN4 antibody partially cross-blocks binding of the anti-human CD9 antibody MM2/57.
Applications Reported:
Flow cytometric analysis.
Applications Tested:
Pre-titrated and validated for flow cytometry using normal human peripheral blood cells. Recommended usage: 5 µL (0.125 µg) per 100 µL test volume. Cell count range: 10⁵–10⁸ cells/test.
Fluorescence Characteristics:
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD9 antigen is a surface glycoprotein expressed on developing B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated T lymphocytes, neurons, and glial cells. It belongs to the tetraspanin family, which spans the membrane four times.
In pre-B cells and platelets, CD9 regulates activation and aggregation via association with integrin CD41/CD61 (GPIIb/GPIIIa). It plays roles in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion. CD9 is essential for MHC II molecule association on dendritic cells, influencing T cell stimulation.
CD9 functions in differentiation, adhesion, and signal transduction, contributing to suppression of cancer cell motility and metastasis, and is considered a metastasis suppressor in solid tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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