Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), FITC, eBioscience
인간 CD8a 분자를 특이적으로 인식하는 단클론 항체로, FITC 형광 표지되어 있습니다. Flow cytometry에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. CD8 T세포 및 NK세포 분석에 적합하며, 488 nm 여기/520 nm 방출 특성을 가집니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test | View 49 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Cynomolgus monkey, Human, Mouse, Rat, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RPA-T8 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10669558 |
Available Conjugate Formats
- Alexa Fluor 532, 561, 660, 700
- APC, APC-eFluor 780
- Brilliant UV 395, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506
- NovaFluor Blue 610-70S, 660-120S
- NovaFluor Red 710, Yellow 610, Yellow 660
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
Description:
The RPA-T8 monoclonal antibody reacts with the human CD8a molecule (32–34 kDa), a cell surface receptor expressed as a heterodimer with CD8β or as a homodimer (CD8α/α). It is present on most thymocytes and a subset of mature T cells and NK cells. CD8 binds MHC class I and associates with tyrosine kinase p56lck, playing a key role in T-cell development and activation.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested for flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (1 µg) per test in a final volume of 100 µL. Cell numbers may vary from 10⁵ to 10⁸ cells/test.
Fluorophore Characteristics:
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It functions as a co-receptor with the T-cell receptor (TCR) for MHC class I molecules. CD8 is expressed mainly on cytotoxic T lymphocytes (CTLs), as well as subsets of NK cells, monocytes, and dendritic cells. Upon binding to MHC-I/peptide complexes, CD8 recruits Lck kinase, leading to lymphokine production, motility, and CTL activation. Activated CTLs are key in pathogen and tumor cell clearance. Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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