Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• NovaFluor Blue 610-70S, 660-120S
• NovaFluor Red 710, Yellow 610, Yellow 660
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

Description:
The RPA-T8 monoclonal antibody reacts with the human CD8a molecule (32–34 kDa), a cell surface receptor expressed as a heterodimer with CD8β or as a homodimer (CD8α/α). It is present on most thymocytes and a subset of mature T cells and NK cells. CD8 binds MHC class I and associates with tyrosine kinase p56lck, playing a key role in T-cell development and activation.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested for flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (1 µg) per test in a final volume of 100 µL. Cell numbers may vary from 10⁵ to 10⁸ cells/test.

Fluorophore Characteristics:
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It functions as a co-receptor with the T-cell receptor (TCR) for MHC class I molecules. CD8 is expressed mainly on cytotoxic T lymphocytes (CTLs), as well as subsets of NK cells, monocytes, and dendritic cells. Upon binding to MHC-I/peptide complexes, CD8 recruits Lck kinase, leading to lymphokine production, motility, and CTL activation. Activated CTLs are key in pathogen and tumor cell clearance. Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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