Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), FITC, eBioscience
인간 CD8a 항원을 인식하는 단클론 항체로, FITC로 표지되어 세포 분석에 적합합니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, T세포 및 NK세포의 CD8 발현 연구에 활용됩니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | 2 |
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | 28 |
| Immunoprecipitation (IP) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT8 (OKT-8) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10609348 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- Functional Grade
- NovaFluor Blue, Red, UV, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
The OKT8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as a heterodimer (CD8αβ) or homodimer (CD8αα). CD8a is found on most thymocytes and a subset of mature T cells and NK cells. It binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a key role in T-cell development and activation.
Preliminary testing shows that OKT8 does not compete with other anti-human CD8 clones (RPA-T8, HIT8a) for binding, suggesting distinct epitopes.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry of normal human peripheral blood cells. Use 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Excitation: 488 nm
Emission: 520 nm
Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It functions as a co-receptor for MHC class I molecules and is crucial for T cell differentiation and activation. CD8 is mainly expressed on cytotoxic T lymphocytes and a subset of NK cells, monocytes, and dendritic cells. Upon MHC-I/peptide complex binding, CD8 recruits Lck kinase, initiating signaling that leads to cytokine production, motility, and cytotoxic T lymphocyte activation. Differentiation of naïve CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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