Thermo Fisher Scientific Phospho-EGFR (Tyr845) Polyclonal Antibody
EGFR Tyr845 인산화 부위를 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF에 사용 가능. Human에 반응하며 Mouse, Rat, Pig에서도 예측 반응. 항원 친화 크로마토그래피로 정제된 액상 형태로, 고농도 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human EGFR (phospho Tyr845) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792951 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%).
- Positive Control: EGF treated A431 cell.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer, and EGFR is the target of both biological and small molecular therapeutics.
EGFR is encoded by the EGFR gene located on chromosome 7 in humans and belongs to the HER/ERbB family of proteins (ERbB2, ERbB3, ERbB4).
Binding of ligands such as EGF and TGF-alpha to the extracellular domain leads to EGFR dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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