Thermo Fisher Scientific Phospho-RIP1 (Ser166) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

• Positive test controls include: HT-29, NIH/3T3
• Immunogen sequence: MWSKL

Target Information

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptor ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor.
Phosphorylates DAB2IP at Ser-728 in a TNF-alpha-dependent manner, activating the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via Lys-63-linked chains enhances communication with downstream signal transducers in the MAPK and NF-kappa-B pathways, mediating gene activation and inflammatory responses.
Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, which is critical for NF-kappa-B activation. Interaction with RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association forms a necrosis-inducing complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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