Thermo Fisher Scientific N-cadherin Polyclonal Antibody
Western blot, IHC, IP 등 다양한 응용에 적합한 N-cadherin 폴리클로날 항체. 인간, 생쥐 등 여러 종에 반응하며 항원 친화 크로마토그래피로 정제됨. 액상 형태로 보존하며 -20°C에서 저장 권장. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunoprecipitation (IP) | 1:25 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Fruit fly, Human, Mouse, Non-human primate, Rat, Zebrafish |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Two synthetic peptides corresponding to two conserved regions of human N-Cadherin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 9 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10985390 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species.
N-cadherin's function depends on its association with the actin-cytoskeleton and interactions between its C-terminal region and cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin.
N-cadherin is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail. It functions during gastrulation and is required for the establishment of left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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