Thermo Fisher Scientific Myelin Basic Protein Recombinant Mouse Monoclonal Antibody (rMBP, 4288)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. MBP-related transcripts are also present in the bone marrow and immune system. These mRNAs arise from the long MBP gene (also called Golli-MBP) that contains three additional exons located upstream of the classic MBP exons.
Alternative splicing from the Golli and MBP transcription start sites gives rise to two sets of MBP-related transcripts and gene products. The Golli mRNAs contain three exons unique to Golli-MBP, spliced in-frame to one or more MBP exons, encoding hybrid proteins with N-terminal Golli amino acid sequence linked to MBP sequence.
The second family of transcripts contains only MBP exons and produces the well-characterized myelin basic proteins. This complex gene structure is conserved among species, suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for function and/or regulation of these genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.