Thermo Fisher Scientific Myelin Basic Protein Recombinant Mouse Monoclonal Antibody (rMBP, 4288)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
• For formalin-fixed tissues, heat sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, then cool at room temperature for 20 min.

Target Information

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. MBP-related transcripts are also expressed in the bone marrow and immune system, derived from the long MBP gene (Golli-MBP) containing three upstream exons. Alternative splicing from Golli and MBP transcription start sites produces two sets of MBP-related transcripts and gene products:

1. Golli-MBP transcripts – Contain three unique exons spliced in-frame with MBP exons, encoding hybrid proteins combining Golli and MBP amino acid sequences.
2. Classic MBP transcripts – Contain only MBP exons, producing the well-characterized myelin basic proteins.

This conserved gene structure suggests that the MBP transcription unit is an integral part of the Golli transcription unit, important for gene regulation and function.

For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.