Thermo Fisher Scientific ATG3 Monoclonal Antibody (OTI2C12), TrueMAB
ATG3 단백질을 표적으로 하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 적합. 고순도 친화 크로마토그래피 정제 제품으로, 리오필라이즈드 형태 및 PBS 버퍼에 8% 트레할로스 포함. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C12 |
| Immunogen | Full length human recombinant protein of human ATG3 produced in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ATG3 (APG3) is a conjugating enzyme essential for APG8 lipidation, a critical step in autophagy initiation.
Autophagy is a process for bulk degradation of cellular proteins via an autophagosomic-lysosomal pathway, important for normal growth control and nutrient preservation under starvation.
ATG3 acts as an E2-like enzyme catalyzing Atg8-phosphatidylethanolamine (Atg8-PE) conjugate formation, which is vital for autophagosome formation.
This process is negatively regulated by TOR through phosphorylation of autophagy protein APG1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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