Thermo Fisher Scientific LIMK1 Monoclonal Antibody (OTI1F1), TrueMAB
LIMK1 단백질을 인식하는 마우스 단클론 항체로, Western blot, ICC/IF, Flow Cytometry에 사용 가능. HEK293T 세포에서 발현된 human LIMK1 단백질로 면역화. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F1 |
| Immunogen | Full length human recombinant protein of human LIMK1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
LIMK1 is a serine/threonine kinase involved in actin cytoskeletal regulation through phosphorylation of cofilin. It also plays a role in brain development. LIM domains are conserved cysteine-rich structures containing two zinc fingers, which mediate protein-protein interactions. LIM kinase-1 and LIM kinase-2 form a small subfamily characterized by two N-terminal LIM motifs and a C-terminal kinase domain. LIMK1 hemizygosity has been linked to cognitive impairment in Williams syndrome.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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