Thermo Fisher Scientific NTF4 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence (recombinant protein):
GVSETAPASR RGELAVCDAV SGWVTDRRTA VDLRGREVEV LGEVPAAGGS PLRQYFFETR CKADNAEEGG PGAGGGGCRG VDRRHWVSEC KAKQSYVRAL TADAQGRVGW RWIRIDTACV CTLLSRTGRA

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human NT-4. Anti-Human NT-4-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hNT-4 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NT-4 (500-P83G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hNT-4.

Western Blot:
To detect hNT-4 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant hNT-4 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P83GBT-1MG is provided as 2 × 500 µg.

Target Information

This gene encodes a member of the neurotrophin family, which regulates survival and differentiation of mammalian neurons. Expression is ubiquitous and less influenced by environmental signals.
Knockouts of other neurotrophins (NGF, BDNF, NT-3) are lethal during early postnatal development, whereas NTF5-deficient mice show minor cellular deficits and develop normally to adulthood.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.