Thermo Fisher Scientific BDNF Polyclonal Antibody, PeproTech
BDNF 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, ELISA, 중화, 면역염색 등에 사용 가능합니다. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관합니다.
- 카탈로그번호
- 500-P84-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific BDNF Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | - |
| Neutralization (Neu) | - | View 5 publications |
| Immunostaining (IS) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human/Murine/Rat BDNF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930803 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human/Murine/Rat BDNF. The anti-BDNF antibody was purified by affinity chromatography using immobilized Human/Murine/Rat BDNF matrix.
Sandwich ELISA:
For detection of Human/Murine/Rat BDNF by sandwich ELISA (100 µL/well antibody solution), use 0.5–2.0 µg/mL of this antibody. In conjunction with PeproTech Biotinylated Anti-Human/Murine/Rat BDNF (500-P84Bt) as a detection antibody, it detects at least 0.2–0.4 ng/well of recombinant BDNF.
Western Blot:
For detection of Human BDNF, use 0.1–0.2 µg/mL of this antibody. Detection limit for recombinant BDNF is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family, synthesized as pre-proBDNF and processed to proBDNF and mature BDNF. Both forms are biologically active and secreted in synaptic vesicles.
BDNF acts via the TrkB receptor and low-affinity p75-NTR receptor, promoting neuronal survival, differentiation, and synaptic regulation.
It plays a key role in memory formation and is reduced in Alzheimer’s and Huntington’s disease. BDNF also contributes to stress response and mood regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.