Thermo Fisher Scientific GRK2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Keep as concentrated solution.
• Predicted reactivity: Mouse (99%), Rat (98%), Pig (99%), Bovine (99%).
• Positive Control: H1299, Molt-4, Raji.
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ADRBK1 (GRK2) is a serine/threonine kinase. Many of the G protein-coupled receptors are known to be phosphorylated by G protein-dependent receptor kinases (GRKs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals.
After ligand binding and interaction with heterotrimeric G proteins, GPCRs (e.g., α2-adrenergic receptor) are phosphorylated by GRKs. Internalization of GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras–Raf–ERK1&2 signaling pathway.
GRK2 activity is tightly controlled by various mechanisms including phosphorylation by kinases such as PKC, Src, and ERK1&2, as well as interaction with multiple proteins. ERK phosphorylates and inactivates GRK2 on serine 670 in a negative feedback mechanism.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.