Thermo Fisher Scientific IKK beta Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Keep as concentrated solution.
• Predicted reactivity: Mouse (80%).
• Positive Control: Jurkat, Raji, K562, THP-1, NCI-H929, DDDDK-tagged IKBKB-transfected 293T.
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Phosphorylation of serine residues on the I-kappa-B proteins by IKK kinases, including IKBKB, marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex.
Inhibitor of nuclear factor kappa-B kinase subunit beta (IKK beta) is a serine kinase that plays an essential role in the NF-kappa-B signaling pathway, which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damage, or other cellular stresses.
IKK beta acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on two critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome.
Free NF-kappa-B is then translocated into the nucleus and activates transcription of genes involved in immune response, growth control, or protection against apoptosis.
Mutations affecting the IKK beta gene can result in Immunodeficiency 15 (IMD15).

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.