Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (PO3.1), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test
• Publications: View 24 publications

Product Specifications

Additional Formats

• Functional Grade
• Request custom conjugation

Product Specific Information

Description:
The PO3.1 monoclonal antibody reacts with mouse CD86 (B7-2), an ~80 kDa surface receptor belonging to the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated by various stimuli such as BCR complex, CD40, and cytokine receptors. It is also expressed by activated mouse T cells and peritoneal cells.

CD86 interacts with T-cell surface molecules CD28 and CD152 (CTLA-4), playing a critical role in T-B cell crosstalk, T cell costimulation, and antibody production. Its rapid upregulation during stimulation supports its role in the primary immune response.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Tested on resting and activated mouse splenocytes
• Recommended use: ≤ 0.125 µg/test (in 100 µL final volume)
• Suitable for 10⁵–10⁸ cells/test
• Optimal titration recommended for specific assay conditions

Fluorescence Characteristics:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD86 is a ligand for CTLA4 and CD28, acting as a costimulatory molecule in T-cell activation during antigen presentation. Insufficient co-stimulation involving CD86/CD80 can induce tolerance. CD86 is a type I membrane protein of the immunoglobulin superfamily, expressed by antigen-presenting cells.

Binding of CD86 with CD28 provides a costimulatory signal for T-cell activation, while interaction with CTLA-4 inhibits T-cell activation and modulates immune response. Alternative splicing results in multiple isoforms of CD86. Dysregulation of CD86 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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