Thermo Fisher Scientific NONO Monoclonal Antibody (OTI5F1), TrueMAB
NONO 단백질을 인식하는 Mouse monoclonal antibody로, WB와 IHC(P)에서 검증됨. Human, Mouse, Dog 반응성. Lyophilized 형태로 제공되며, PBS buffer에 trehalose 포함. 연구용으로 전사 조절 및 RNA 스플라이싱 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5F1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 184–385 of human NONO produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The NONO gene encodes an RNA-binding protein involved in multiple nuclear processes such as transcriptional regulation and RNA splicing.
A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma.
Alternatively spliced transcript variants have been described, and pseudogenes exist on Chromosomes 2 and 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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