Thermo Fisher Scientific PECAM1 Monoclonal Antibody (OTI1F9), TrueMAB
인간 PECAM1(CD31)을 인식하는 단클론 항체로, Western blot 및 IHC(파라핀) 실험에 적합. 마우스 IgG1 아이소타입, 비결합형 상태로 공급. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 안정적 보관 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F9 |
| Immunogen | Full length human recombinant PECAM1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor that regulates T and B cell signaling through ITIM motifs. It is ubiquitously expressed within the vascular compartment, mainly at cell junctions. The N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions.
CD31 plays diverse roles in:
- Integrin-mediated adhesion
- Transendothelial migration
- Angiogenesis
- Apoptosis
- Immunoreceptor signaling regulation
- Autoimmunity and thrombosis control
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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