Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Ultra Violet 395, eBioscience
Armenian hamster IgG 동형 대조 항체로, Brilliant Ultra Violet 395 형광체가 결합된 단클론 항체입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 비특이적 결합 확인용 음성 대조로 사용됩니다. 4°C 암소 보관, 연구용 전용.
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Applications and Tested Dilution
Flow Cytometry (Flow)
- Assay-dependent
Control (Ctrl)
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBio299Arm |
| Conjugate | Brilliant Ultra Violet™ 395 |
| Excitation / Emission Max | 347 / 399 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925287 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
The eBio299Arm monoclonal antibody is used as an isotype control immunoglobulin.
Reported for use in flow cytometric analysis.
Tested on normal human peripheral blood and mouse splenocytes.
Use at the same concentration as the experimental antibody.
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is designed for cytometers equipped with an ultraviolet (355 nm) laser.
Ensure your instrument can detect this fluorochrome.
When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
Isotype control antibodies serve as negative controls to distinguish non-specific background from specific antibody signal.
They are useful in:
- Flow cytometry
- Chromatin immunoprecipitation (ChIP)
- Immunohistochemistry
- Gel shift assays
Isotype controls should match the species and isotype of the primary antibody for accurate comparison.
For directly labeled antibodies, use an isotype control conjugated with the same fluorochrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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