Thermo Fisher Scientific GAPDH Polyclonal Antibody
GAPDH 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합합니다. 인간, 생쥐, 닭 등 여러 종에 반응하며, 항원 친화 크로마토그래피로 정제된 고품질 항체입니다.
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- PA116777
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Thermo Fisher Scientific GAPDH Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | View 13 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 2 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Non-human primate, Rat |
| Published Species | Hamster, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Residues between 150–200 of human Glyceraldehyde-3-Phosphate Dehydrogenase (GeneID 2597) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_568552 |
Product Specific Information
- Antigen retrieval with sodium citrate buffer (pH 6.0) is recommended prior to immunostaining paraffin tissues.
- Suggested positive control: antigen standard for GAPDH (transient overexpression lysate).
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis. It forms a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH participates in DNA replication, DNA repair, nuclear RNA export, membrane fusion, and microtubule bundling. It plays essential roles in apoptosis, neurodegenerative diseases, and cancer-related processes. GAPDH interacts with proteins such as amyloid precursor protein (linked to Alzheimer’s disease) and Huntingtin (linked to Huntington’s disease), as well as actin and tubulin.
Due to its high expression in most tissues, GAPDH is widely used as a protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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