Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), APC-eFluor 780, eBioscience
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카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
47-5893-82 | - | Thermo Fisher Scientific 47-5893-82 KLRG1 Monoclonal Antibody (2F1), APC-eFluor 780, eBioscience 100 ug pk | 재고문의 | pk | 564,000원 | - | 620,400원 | |
47-5893-80 | - | Thermo Fisher Scientific 47-5893-80 KLRG1 Monoclonal Antibody (2F1), APC-eFluor 780, eBioscience 25 ug pk | 재고문의 | pk | 241,000원 | - | 265,100원 |
다른 상품 둘러보기
Applications
Tested Dilution
Publications
Flow Cytometry (Flow)
0.5 µg/test
View 17 publications 17 publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Syrian hamster / IgG
Class
Monoclonal
Type
Antibody
Clone
2F1
Conjugate
APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780
View additional formats
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- APC
- Biotin
- Brilliant UV 395
- Brilliant UV 496
- Brilliant UV 563
- Brilliant UV 615
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 711
- eFluor 450
- eFluor 506
- FITC
- Functional Grade
- Pacific Orange
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Excitation/Emission Max
756/785 nm View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573987
Product Specific Information
Description: This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.
Applications Reported: This 2F1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 2F1 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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