Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (8B.2C12), APC-eFluor 780, eBioscience

Applications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• eFluor 450
• Functional Grade
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 8B.2C12 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. CD366 is a type I transmembrane protein with an immunoglobulin and mucin-like domain extracellularly and a tyrosine phosphorylation motif in its cytoplasmic portion. It is selectively expressed by differentiated CD4⁺ Th1 and CD8⁺ Tc1 cells, but absent on Th2 and Tc2 cells. Other hematopoietic cells such as naive T cells, B cells, macrophages, and dendritic cells do not express CD366 at the protein level.

Expression of CD366 increases during late T cell differentiation on Th1 cells, suggesting a role in transport or effector function rather than differentiation. In EAE models, CD366 expression is high in CNS T cells during disease onset, while peripheral expression remains low. Administration of 8B.2C12 induces a hyperacute disease phenotype, indicating CD366 involvement in macrophage activation and autoimmune responses. The Tim gene family may play a key role in regulating autoimmunity and allergies.

The 8B.2C12 antibody binds strongly to the BALB/c allele of CD366 but weakly to the C57Bl/6 allele.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Optimal titration is recommended.

Fluorochrome Information:
APC-eFluor 780 emits at 780 nm and is excited by the Red laser (633–647 nm). Ensure the instrument supports this fluorochrome.

Light Sensitivity:
This tandem dye is light-sensitive. Protect from light during storage and staining.

Fixation:
Samples can be fixed in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal signal loss.

Excitation: 633–647 nm
Emission: 780 nm
Laser: Red Laser

Target Information

TIM3 (HAVCR2, T-cell immunoglobulin and mucin-domain containing-3) is a 281 amino acid, type I Th1-specific cell surface glycoprotein expressed on terminally differentiated CD4⁺Th1 and CD8⁺Tc1 cells. It includes an IgV-like domain, a mucin-like domain, and a conserved tyrosine phosphorylation motif. TIM3 is involved in macrophage activation, autoimmune disease induction, and immune tolerance regulation. It plays a role in experimental autoimmune encephalomyelitis (EAE) and enhances disease severity in mouse models.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지