Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr319, Tyr352) Monoclonal Antibody (n3kobu5), PE, eBioscience

Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr319, Tyr352) Monoclonal Antibody (n3kobu5), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.03 µg)/test
• Publications: View 3 publications

Product Specifications

Available Formats

• APC
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This n3kobu5 monoclonal antibody recognizes human and mouse ZAP-70 and SYK when phosphorylated on Y319 and Y352, respectively.
ZAP-70 and SYK are members of the SYK protein tyrosine kinase family, activated by Src family PTKs. Phosphorylation of ZAP-70 Y319 by Lck is essential for TCR-dependent signaling, while SYK Y352 phosphorylation by Fyn/Lyn is critical for BCR signaling and B cell development.

Specificity of the n3kobu5 clone was validated by ELISA, flow cytometry, and western blotting.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and tested on stimulated normal human peripheral blood cells at 5 µL (0.03 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).

Staining Protocols

1. Protocol A (Two-step, cytoplasmic proteins): Flexible detection of surface and intracellular proteins.
2. Protocol B (One-step, nuclear proteins): Recommended for transcription factor staining.
3. Protocol C (Two-step, Fixation/Methanol): Best for phospho-specific signaling discrimination; limited surface protein staining.

Detailed protocols are available in the BestProtocols® Section.

Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

ZAP-70 is a cytosolic protein tyrosine kinase of the Syk family, expressed in T-cells and NK cells, required for TCR activation.
Upon TCR engagement, ZAP-70 is phosphorylated on tyrosines 292, 315, and 319.
Phosphorylation of 315 and 319 enhances signaling, while 292 attenuates activation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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