Thermo Fisher Scientific Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), PE, eBioscience
인간 및 마우스의 인산화된 S6 단백질(Ser235/Ser236)을 인식하는 cupk43k 단클론 항체로, PE 형광 표지되어 있습니다. 세포내 염색 및 유세포분석에 최적화되어 있으며, 5 µL/test로 사용합니다. 성장 신호 및 세포주기 연구에 적합합니다.
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Thermo Fisher Scientific Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 9 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | cupk43k |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572666 |
Additional Formats
- Unconjugated
- APC
- eFluor 450
- PE-Cyanine7
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
This cupk43k monoclonal antibody recognizes human and mouse ribosomal protein S6 when phosphorylated on serine 235 (human) and serine 236 (mouse). Ribosomal protein S6 is a component of the 40S ribosomal subunit and is phosphorylated following stimulation by growth factors, tumor-promoting agents, or mitogens. Phosphorylation by p70S6K and PKDCD upregulates translation of proteins involved in cell cycle entry, while dephosphorylation occurs upon growth arrest.
The specificity of this antibody was confirmed by western blotting.
Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and validated for intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. Recommended usage is 5 µL (0.125 µg) per test (defined as staining a sample in 100 µL volume).
Protocol Guidance:
- Protocol A (Two-step, cytoplasmic): Allows detection of surface and intracellular proteins.
- Protocol B (One-step, nuclear): Recommended for transcription factors with surface and phosphorylated intracellular proteins.
- Protocol C (Two-step, Fixation/Methanol): Best for phospho-specific signaling discrimination; limited surface staining capability.
Detailed protocols are available in the Staining Intracellular Antigens for Flow Cytometry Protocol section under BestProtocols®.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Ribosomal protein S6 is part of the 40S subunit and a member of the S6E family. It is the major substrate for ribosomal protein kinases, phosphorylated at multiple serine residues in response to growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest, and S6 may regulate cell growth and proliferation through selective translation of specific mRNA classes. Multiple processed pseudogenes of this gene exist throughout the genome.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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