Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), eFluor 450, eBioscience
마우스 NK1.1 항원을 인식하는 PK136 단클론 항체로, eFluor 450으로 표지되어 있습니다. Flow cytometry 분석에 적합하며 405/445 nm 파장에서 작동합니다. C57BL/6 등 NK1.1 발현 마우스주 분석에 유용하며, 4°C 암소 보관이 권장됩니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), eFluor 450, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 61 publications |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PK136 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2043878 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 661 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- FITC
- Functional Grade
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer (NK) cells and a subset of T cells in NK1.1 mouse strains including C57BL and NZB.
Common laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1. For these strains, the DX5 monoclonal antibody (Product # 14-5971) should be used.
Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 shows coexpression of both markers by most cells and presence of DX5⁺PK136⁻ and DX5⁻PK136⁺ populations.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on C57Bl/6 splenocytes at ≤1 µg/test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test).
Recommendation: Titrate antibody for optimal performance.
eFluor® 450 is an alternative to Pacific Blue®, excited by a 405 nm violet laser and emitting at 445 nm. Ensure instrument compatibility.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
NK1.1 (Killer cell lectin-like receptor subfamily B, member 1; KLRB1; NKR-P1A; CD161) is a marker for natural killer (NK) cells, which mediate cytotoxicity and secrete cytokines upon immune stimulation.
KLRB1 protein belongs to the C-type lectin superfamily and contains extracellular, transmembrane, and cytoplasmic domains.
NKR-P1A (CD161) recognizes Lectin Like Transcript-1 (LLT1) as its functional ligand and is classified as a type II membrane protein with an external C-terminus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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