Thermo Fisher Scientific PPAR alpha/gamma Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: I(484) K K T E T D M S L H P L L Q(498)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2165594

Product Specific Information

PA3-820 detects peroxisome proliferator activated receptor (PPAR) gamma 2 from human, mouse, and rat tissues and cells.

PA3-820 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~55 kDa protein representing PPAR gamma2 from 3T3-L1 cell lysate.

PA3-820 immunizing peptide corresponds to amino acid residues 484-498 from rat PPAR gamma2. This sequence is completely conserved in bovine, chicken, human, mouse, pig and rat PPAR gamma1, PPAR alpha and NUC1.

Target Information

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR`s can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.