Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Polyclonal Antibody
Phospho-Histone H3 (Ser10) 인산화 형태를 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, IP, ChIP 등 다양한 응용에 적합. Human, Mouse, Rat, Zebrafish 시료 반응. 항원 친화 크로마토그래피로 정제된 고품질 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | 1 publication |
| Immunohistochemistry - Free Floating (IHC (Free)) | 1:100–1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
| ChIP assay (ChIP) | Assay-dependent | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H3S10ph (phospho Ser10) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.19 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792646 |
Product Specific Information
- Keep as concentrated solution.
- Positive Control: HeLa, HeLa (100 ng/mL Nocodazole treatment for 24 hr), U2OS (100 ng/mL Nocodazole treatment for 24 hr), 4T1 (100 ng/mL Nocodazole treatment for 24 hr), Rat2 (100 ng/mL Nocodazole treatment for 24 hr).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Along with other core histone proteins, it binds DNA to form the nucleosome structure. Histones are crucial in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 undergoes various post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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