Thermo Fisher Scientific SMAD2 Monoclonal Antibody (5G7)
SMAD2 단백질 검출용 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 5G7). Western blot, IHC, ICC/IF, Flow Cytometry, ELISA에 적합하며 Human 시료에 반응. 비결합형 액상 형태로 4°C 단기 보관, -20°C 장기 보관 권장.
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Thermo Fisher Scientific SMAD2 Monoclonal Antibody (5G7)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | 1 publication |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5G7 |
| Immunogen | Purified recombinant fragment of human SMAD2 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153038 |
Product Specific Information
MA5-15877 targets SMAD2 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
The immunogen is a purified recombinant fragment of human SMAD2 expressed in E. coli.
MA5-15877 detects SMAD2 with a predicted molecular weight of approximately 52 kDa.
Target Information
SMAD2 (MADH2, MAD2) regulates multiple cellular processes such as cell proliferation, apoptosis, and differentiation.
It interacts with TGF-beta receptors through the SMAD anchor for receptor activation, a central event in TGF-beta signaling.
Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by ERK1 enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin.
Upon TGF-beta signaling, SMAD2 is phosphorylated by TGF-beta receptors, leading to dissociation from SARA and association with SMAD4, enabling nuclear translocation and transcriptional repression.
SMAD2 can also be phosphorylated by activin type 1 receptor kinase, mediating activin signaling.
Alternatively spliced transcript variants encoding SMAD2 have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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