Thermo Fisher Scientific CD44H Monoclonal Antibody (OX49), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Rat

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OX49

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11150786

Product Specific Information

Description: This OX49 monoclonal antibody recognizes rat CD44H, which is also known as homing-associated cell adhesion molecule (H-CAM) or phagocytic glycoprotein-1 (Pgp-1). This isoform of CD44 is the cell surface receptor for hyaluronate, a component of the extracellular matrix. CD44H is highly expressed on hematopoietic and non-hematopoietic cells, and exists as multiple splice variants. CD44H plays a critical role in lymphocyte activation and homing.

Applications Reported: This OX49 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX49 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.