Thermo Fisher Scientific PD-L1 Monoclonal Antibody (OTI9E12), TrueMAB
Human PD-L1 단백질(aa 19–239)을 인식하는 mouse IgG1 단일클론 항체. Western blot, Flow cytometry, ELISA 등 다양한 분석에 적합. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. 종양 면역회피 연구에 활용 가능.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500 | |
| Flow Cytometry (Flow) | Assay-dependent | |
| ELISA | Assay-dependent | |
| Luminex (LUM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9E12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 19–239 of human PD-L1/CD274 produced in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a member of the B7 family. PD-L1 binds to its receptor PD-1, which negatively regulates T-cell receptor (TCR) signaling. Binding to PD-1 decreases TCR-mediated proliferation and cytokine production. PD-L1 expression is abundant in many murine and human cancers and can be upregulated by IFN-gamma stimulation, suggesting its role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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