Thermo Fisher Scientific Sulfotyrosine Monoclonal Antibody (7C5), PE
Sulfotyrosine 단백질의 검출에 특이적인 단클론 항체. PE 형광 표지로 유세포 분석 및 형광 이미징에 적합. Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. 단백질 G 정제 및 보존제 미함유. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7C5 |
| Immunogen | Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932290 |
Additional Formats
- Unconjugated (MA5-27551)
- APC (MA5-45833)
- FITC (MA5-45834)
- PerCP (MA5-45835)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45836 was sufficient for detection of Sulfotyrosine in 0.5 µg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
Target Information
Sulfotyrosine is involved in protein-protein interactions. Sulfotyrosines are present in all multicellular eukaryotes, but unicellular eukaryotes such as yeast and prokaryotes are not capable of tyrosine sulfation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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