Thermo Fisher Scientific Dityrosine Monoclonal Antibody (10A6), PE
Dityrosine 변형 단백질을 특이적으로 인식하는 PE 결합 단클론 항체. Western blot, ICC/IF, Flow cytometry, ELISA에 적합. 단백질 산화 스트레스 연구에 활용 가능. 565/576 nm 형광 특성, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10A6 |
| Immunogen | Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932286 |
Available Formats
- Unconjugated (Cat. MA5-27566)
- APC (Cat. MA5-45829)
- FITC (Cat. MA5-45830)
- PerCP (Cat. MA5-45831)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45832 was sufficient for detection of dityrosine in 1 µg of dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for dityrosine-modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine-modified proteins.
Target Information
Dityrosine may be a natural component of protein structure, a product of environmental stress, or a result of in vitro protein modification.
It is involved in reactions that produce tyrosyl radicals, which can couple to yield dityrosine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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