Thermo Fisher Scientific UPF1 Recombinant Rabbit Monoclonal Antibody (ARC1268)
UPF1 단백질을 인식하는 토끼 유래 재조합 단클론 항체. Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. HEK293 세포 발현, 액상 형태, 친화 크로마토그래피 정제. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1268 |
| Immunogen | A synthetic peptide corresponding to amino acids 1–100 of human UPF1/RENT1 (Q92900) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849534 |
Product Specific Information
Immunogen sequence:
MSVEAYGPSS QTLTFLDTEE AELLGADTQG SEFEFTDFTL PSQTQTPPGG PGGPGGGGAG GPGGAGAGAA AGQLDAQVGP EGILQNGAVD DSVAKTSQLL
Target Information
Upf1 is an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons. It functions as an ATP-dependent RNA helicase in the cytoplasm and is also a component of cytoplasmic P-bodies. Upf1 phosphorylation mediates translational repression associated with NMD, facilitating mRNA accessibility to the NMD machinery. Other NMD components, Upf2 and Upf3, exhibit perinuclear and nucleocytoplasmic localization, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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