Thermo Fisher Scientific Histone H3.3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25-0.5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Chicken, Fruit fly, Horse, Human, Marsupial, Mouse, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A portion of amino acids 100-150 of human Histone H3.3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2540812

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.