Thermo Fisher Scientific BMI-1 Recombinant Rabbit Monoclonal Antibody (ARC0376)
BMI-1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 ELISA에 사용 가능. HEK293 세포에서 발현되었으며, 인간과 랫트 시료에 반응. 고순도 친화 크로마토그래피 정제, 안정한 PBS/glycerol 완충액에 보존.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0376 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 227–326 of human Bmi1 (P35226) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.52 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849094 |
Product Specific Information
Immunogen sequence:
VRPTCKRMKI SHQRDGLTNA GELESDSGSD KANSPAGGIP STSSCLPSPS TPVQSPHPQF PHISSTMNGT SNSPSGNHQS SFANRPRKSS VNGSSATSSG
Target Information
Component of the Polycomb group (PcG) multiprotein PRC1 complex, required to maintain transcriptional repression of many genes, including Hox genes, during development. The PRC1 complex acts through chromatin remodeling and histone modification, mediating monoubiquitination of histone H2A at Lys-119, leading to heritable changes in chromatin expressibility. Within PRC1, BMI-1 is essential for stimulating the E3 ubiquitin-protein ligase activity of RNF2/RING2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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